of bar-coded 16s rrna gene fragments  (Pyrosequencing Inc)

Journal: International Journal of Molecular Sciences

Article Title: The Complex Interplay between Vaginal Microbiota, HPV Infection, and Immunological Microenvironment in Cervical Intraepithelial Neoplasia: A Literature Review

doi: 10.3390/ijms23137174

Figure Lengend Snippet: Characteristics of studies exploring the association of HPV infection and cervical preinvasive disease to the vaginal microbiome.

Article Snippet: Mengying Wu et al., 2020 [ ] , Shanghai, China; Obstetrics and Gynecology Hospital of Fudan University , 69 premenopausal, non-pregnant patients, prospective case–control study , Scraping from posterior vaginal fornix Deep sequencing of bar-coded 16S rRNA gene fragments (V3–4) using Illumina MiSeq , 31 normal, 22 LSIL, 16 HSIL , − CST II has lower level of Lactobacillus spp. and higher level of strictly anaerobic organisms ( Gardnerella , Atopobium , and Prevotella ) − Prevotella and Streptococcus are increased in HSIL detection − CIN converts the vaginal bacterial community structure from CSTs IV to II − Microbiota diversity is more marked in CST types II and IV ( p < 0.001), above all in type II − Enrichment in the Delftia genus is found in the LSIL and HSIL groups.

Techniques: Infection, Sampling, Microscopy, Immunocytochemistry, Sequencing, DNA Extraction, Hybridization, Marker, Amplification